Journal: Journal of Oncology

Article Title: Cell-Permeable Bak BH3 Peptide Induces Chemosensitization of Hematologic Malignant Cells

doi: 10.1155/2020/2679046

Figure Lengend Snippet: 3D structure of cell-permeable Bak BH3 peptide (PETK) and expression of the Bcl-2 family proteins in hematologic malignant cells. (a) The structure is characterized by having two α -helices connected by a loop. The folding and packing allow the visualization of a structural domain of the bHLH type: a minor helix (Bak BH3 peptide = K) that folds towards the major basic helix (Antennapedia fusogenic peptide = PE) due to the flexibility of the loop (Tag peptide = T); its strong negative charge directs the packaging of the polypeptide. (b) Expression of the antiapoptotic proteins (Mcl-1, Bcl- XL , and Bcl-2) and proapoptotic proteins (Bak and Bax) in Ramos (NHL) and CCRF-CEM (ALL) cells. Proteic extracts of hematologic malignant cells (40 μ g per well) were analyzed by Western Blot using the anti-Mcl-1, anti-Bcl- XL , anti-Bcl-2, anti-Bak, anti-Bax, and anti- β -tubulin antibodies. Goat anti-rabbit IgG antibody (IRDye® Odyssey) was used as a secondary antibody.

Article Snippet: Based on that aim, Ramos and CCRF-CEM cells were treated for 6 hrs with the PET, KT, and PETK peptides at 20 μ M and with the X and PEX peptides at 5 μ M (the peptide dose was decreased to underscore the synergistic effect on the induction of cell death in tumor cells), in the presence and in the absence of subtoxic doses of vincristine 80 nM, a commonly used drug in the treatment of hematological malignancies [ ]. shows that the combination of the cell-permeable Bak BH3 peptide (PETK) with the cell-permeable Bax BH3 peptide (PEX) eliminated 51% of the Ramos cells and 68% of the CCRF-CEM cells; as expected, in the presence of vincristine, cell death increased significantly to 71% in Ramos cells and 82% in CCRF-CEM cells.

Techniques: Expressing, Western Blot

Journal: Journal of Oncology

Article Title: Cell-Permeable Bak BH3 Peptide Induces Chemosensitization of Hematologic Malignant Cells

doi: 10.1155/2020/2679046

Figure Lengend Snippet: The combination of cell-permeable Bak and Bax BH3 peptides increases cell death of hematologic malignant cell lines. (a) Ramos and CCRF-CEM cells were incubated for 12 hrs with 20 μ M of the PETK, PET, KT, X, and PEX peptides and the PETK and PEX combination. CDDP 40 μ M was used as a positive control. (b) Peripheral blood mononuclear cells (PBMC) were incubated for 12 hrs with 20 μ M of the PETK, PET, KT, X, and PEX peptides and with the PETK and PEX combination. CDDP 40 μ M was used as a positive control. In both cases, cell viability was determined with MTT assays. The graphs represent the value of three independent assays. The ANOVA test was performed with Bonferroni post hoc analysis to determine the difference between groups. ∗ p < 0.05.

Article Snippet: Based on that aim, Ramos and CCRF-CEM cells were treated for 6 hrs with the PET, KT, and PETK peptides at 20 μ M and with the X and PEX peptides at 5 μ M (the peptide dose was decreased to underscore the synergistic effect on the induction of cell death in tumor cells), in the presence and in the absence of subtoxic doses of vincristine 80 nM, a commonly used drug in the treatment of hematological malignancies [ ]. shows that the combination of the cell-permeable Bak BH3 peptide (PETK) with the cell-permeable Bax BH3 peptide (PEX) eliminated 51% of the Ramos cells and 68% of the CCRF-CEM cells; as expected, in the presence of vincristine, cell death increased significantly to 71% in Ramos cells and 82% in CCRF-CEM cells.

Techniques: Incubation, Positive Control

Journal: Journal of Oncology

Article Title: Cell-Permeable Bak BH3 Peptide Induces Chemosensitization of Hematologic Malignant Cells

doi: 10.1155/2020/2679046

Figure Lengend Snippet: The combination of cell-permeable BH3 peptides enhances chemotherapy sensitivity in hematologic malignant cell lines. Ramos and CCRF-CEM cells were incubated for 6 hrs with 20 μ M of the PEKT, PET, and KT peptides and 5 μ M of the X and PEX peptides and the combination of PEXT and PEX. Vincristine 80 nM was the chemotherapeutic agent. In both cases, cell viability was determined with MTT assays. The graphs represent the values of three independent assays. The ANOVA test was performed with Bonferroni post hoc analysis to establish the difference between groups. ∗ p < 0.05.

Article Snippet: Based on that aim, Ramos and CCRF-CEM cells were treated for 6 hrs with the PET, KT, and PETK peptides at 20 μ M and with the X and PEX peptides at 5 μ M (the peptide dose was decreased to underscore the synergistic effect on the induction of cell death in tumor cells), in the presence and in the absence of subtoxic doses of vincristine 80 nM, a commonly used drug in the treatment of hematological malignancies [ ]. shows that the combination of the cell-permeable Bak BH3 peptide (PETK) with the cell-permeable Bax BH3 peptide (PEX) eliminated 51% of the Ramos cells and 68% of the CCRF-CEM cells; as expected, in the presence of vincristine, cell death increased significantly to 71% in Ramos cells and 82% in CCRF-CEM cells.

Techniques: Incubation